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Encompassing Amino Acids: 367-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: PLK1 is a member of the Polo-Like Kinase family that localize to centrosomes or spindle pole bodies and undergo dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics. PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death.
Description: Human Plk1 polo box domain (GenBank Accession No. NM_005030), a.a. 367-end with N-terminal GST-tag, MW=53 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_005030
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P53350
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Garcia lvarez et al., Acta Cryst. (2006). F62, 372-375.
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