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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200µM ATP, 0.01% Brij-35 and 2 µM substrate (SER/THR 16, Invitrogen) at room temperature for 1 hour.
Background: PLK2 or Polo-like kinase 2 is a serum-inducible kinase that is a member of the Polo family of serine/threonine protein kinases which play an essential role in normal cell division. PLK2 phosphorylates the P4.1-associated protein (CPAP), a human homologue of SAS-4, in procentriole formation during the centrosome cycle. PLK2 may also play a role as a tumor suppressor since it interacts with TSC1 protein which in turn inhibits mTOR signaling, tumor growth and chemosensitivity under hypoxic conditions. Human lung tumor cells deficient in PLK2 grow larger than control tumors, and PLK2 interacts with endogenous TSC1 protein in these tumors.
Description: Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST-tag, expressed in a baculovirus infected Sf9 cell expression system. Theoretical MW is 107 kDa.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Genbank: NM_006622
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9NYY3
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of substrate per minute at pH 7.5 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Syed, N. et al., Blood. 2006 Jan 1,107(1):250-6.
2. Eckerdt, F. et al., Oncogene. 2005 Jan 10,24(2):267-76.
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