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Encompassing Amino Acids: 58-339
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200µM ATP, 0.01% Brij-35 and 2 µM substrate (SER/THR 16, Invitrogen) at room temperature for 1 hour.
Background: PLK3 or Polo-like kinase 3 is a cytokine-inducible kinase that is a member of the Polo family of serine/threonine protein kinases. PLK3 contains both a catalytic domain and a putative regulatory domain and plays an important role in the regulation of cell cycle progression and tumorigenesis. PLK3 is closely associated with centrosomes in several human cell lines and its localization is dependent on the integrity of microtubules. Hence, PLK3 is also involved in regulating microtubule dynamics and centrosomal function.
Description: Human Plk3 (GenBank Accession No. NM_004073), (a.a. 58-339) with N-terminal His-tag, MW=36.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Source: Baculovirus infected Sf9 cells Purity:≥80% by SDS-PAGE. Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, 50% glycerol and 1 mM DTT.
Genbank: NM_004073
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9H4B4
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of substrate per minute at pH 7.5 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Li, Z., et al., J. Biol. Chem. 280 (17), 16843-16850 (2005).
2.Xie,S., et al., Oncogene 23 (21), 3822-3829 (2004).
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