Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 1-836
Applications: Useful for studying enzyme kinetics and screening small molecule inhibitors.
Assay Conditions: 10 µl of varying amounts of PLK4 was mixed with 5 µl of 1 mg/ml MBP protein substrate and 5 µl of distilled water. Reaction was initiated by adding 5 µl of 250 µM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.
Description: Human polo-like kinase 4(PLK4), also known as serine/threonineprotein kinase 18 (STK18), SAK, GenBankAccession No. NM_014264, a.a. 1-836 withan N-terminal GST-tag, expressed in abaculovirus-infected Sf9 cell expressionsystem. MW = 140 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutatione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Genbank: NM_014264
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O00444
Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Kleylein-Sohn, J. et al., Dev. Cell. 2007; 13: 190-202.
2. Ko, M.A. et al., Nature Genet. 2005; 37: 883-888.
English
Deutsch
