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Applications: Useful for studying protein binding and screening small molecular inhibitors
Assay Conditions: PLPro (SARS-CoV-2) was diluted with DUB reaction buffer and incubated at room temp for 30 minutes. Plate read using KinaseGlo (Promega, #E7031).
Description: SARS-Cov-2 papain-like protease (PLPro), part of a large replicase polyprotein 1ab (E1564-Y1882), GenBank Accession No. QHD43415, with a N-terminal His-tag, expressed in an E. coli expression system. MW=38 kDa.
Formulation: 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol and 115 mM Imidazole.
Genbank: QHD43415
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-tag
Uniprot: P0DTD1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wu, F., et al., Nature. 2020; 579:265-269.
2. Baez-Santos, Y., et al., Antiviral Res. 2015; 115: 21-38.
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