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Encompassing Amino Acids: full length
Applications: Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done using BIOMOL GREEN Reagent. Briefly, 5 µl of varying amounts of PP1A was mixed with 10 µl of buffer containing 0.2% 2-mercaptoethanol and 65 ng/µl BSA and 5 µl of 1 µg/µl Thr-phosphopeptide synthetic substrate (KRT(p)IRR). Reaction was incubated at 37°C for 15 min. before adding 100 µl of BIOMOLE Green Reagent and incubating at room temp. for30 min. Absorbance was read at 650 nm.
Description: Recombinant humanserine/threonine-protein phosphatasePP1-alpha catalytic subunit (PP1A), alsoknown as PPP1CA and PPP1A, GenBankAccession No. NM_002708, full-lengthwith an N-terminal GST tag andexpressed in a baculovirus-infected Sf9insect cell expression system. MW = 62kDa.
Format: Aqueous buffer solution.
Formulation: 50 mM Tris-HCl, pH 7.5, 150mM NaCl, 10 mM glutathione, 0.1 mM EDTA,0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Genbank: NM_002708
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST tag
Uniprot: P62136
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Cohen, P. et al., J. Biol. Chem. 1989; 264 21435-21438.
2. Genoux, D. et al., Nature. 2002; 418: 970- 975.
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