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Encompassing Amino Acids: 2-309 (PP2A-α); 2-589 (PP2AR)
Applications: Useful for the study of enzyme kinetics and for inhibitor screening.
Assay Conditions: Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 3 mM DTT, 10 mM pNPP and PP2A. Assay was done at 37°C for 40 min, and the reaction progress was measured at 405 nm. Specific activity was calculated based on pNPP= 18,000 M-1cm-1.
Description: Protein complex of humanprotein phosphatase 2A catalytic subunit (PP2A), also known as TPD3, RP-C,PP2Ac, and PPP2CA (GenBank AccessionNM_002715, a.a. 2-309 with C-terminal Histag)and human protein phosphatase 2Aregulatory subunit (PP2AR) (GenBankAccession NM_014225, a.a. 2-589 with NterminalGST-tag). Co-expressed in abaculovirus-infected Sf9 cell expressionsystem. MW = 36 kDa (PP2A) and 92 kDa(PP2AR).
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 20% glyceroland 16 mM glutathione
Genbank: NM_002715 (PP2A-α); NM_014225 (PP2AR)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P67775; P30153
Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Van Hoof, C. et al., Genomics. 1995; 28:261-272.
2. Chao, Y. et al., Molec. Cell. 2006; 23:535-546.
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