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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: PRKX is a serine/threonine protein kinase that is closely related to the catalytic subunit of the cAMP-dependent protein kinase. PRKX regulates epithelial morphogenesis during mammalian kidney development and PRKX expression markedly activates migration of cultured renal epithelial cells in the presence of cAMP. Aberrant adult kidney expression of PRKX is found in autosomal dominant polycystic kidney disease (1). PRKX is involved in macrophage and granulocyte maturation. Abnormal recombination between PRKX and a related pseudogene on chromosome Y is a frequent cause of sex reversal disorder in XX males and XY females (2).
Description: Recombinant human PRKX, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_005044
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P51817
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Li, X. et al: PRKX, a phylogenetically and functionally distinct cAMP-dependent protein kinase, activates renal epithelial cell migration and morphogenesis. Proc. Nat. Acad. Sci. vol. 99, 9260-9265, 2002.
2. Klink, A. et al: The human protein kinase gene PKX1 on Xp22.3 displays Xp/Yp homology and is a site of chromosomal instability. Hum. Molec. Genet. vol. 4, 869-878, 1995.
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