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Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: PRMT1 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PRMT1 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT1 activity for screening and profiling applications. The key to the PRMT1Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated R3residue of Histone H4. With this kit, only three simple steps are required formethyltransferase detection. First, S-adenosylmethionine is incubated with a samplecontaining assay buffer and methyltransferase enzyme. Next, primary antibody is added.Finally, the strip plates are treated with an HRP-labeled secondary antibody followed byaddition of the HRP substrate to produce chemiluminescence that can then be measuredusing a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with 8-well strips precoated with histone H4 peptide substrate, reagents, and purified PRMT1 enzyme for 96 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.
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