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Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Contraindications: Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.
Description: The PRMT3 Homogeneous Assay Kit is designed to measure the activity of PRMT3 for screening and profiling applications, using AlphaLISA® technology. Thekey to the PRMT3 Homogeneous Assay Kit is a highly specific antibody that recognizesmethylated substrate. With this kit, only three simple steps on a microtiter plate arerequired for methyltransferase detection. First, a sample containing PRMT3 enzyme isincubated with the biotinylated substrate for one hour. Next, acceptor beads and primaryantibody are added, then donor beads, followed by reading the Alpha-counts.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The PRMT3 Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H4 peptide substrate, primary antibody, methylation assay buffer, and purified PRMT3 for 384 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon S.C., et al. 2005. Genome Biology 6:227.
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