Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 2-end (both)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 10-100 ng methyltransferase PRMT5) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
Background: Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
Description: Human PRMT5 (GenBank Accession No. NM_006109), a.a. 2-end, with N-terminal FLAG-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), a.a. 2-end, with N-terminal His-tag, MW=37 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, 80 ug/ml Flag
Peptide, and 20% Glycerol.
Genbank: NM_006109 (PRMT5), NM_024102 (MEP50)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag (PRMT5), N-terminal His-tag (MEP50)
Uniprot: O14744
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zhao Q. et al., J. Nat. Struct. Mol. Biol. 16 (3), 304-311 (2009).
2. Wang L et al., Mol. Cell. Biol. 28 (20), 6262-6277 (2008).Application Reference(s):
1.
Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and Its Substrate RNA Splicing Factor SF3B2 (2015)
2. Coronin 2A (CRN5) expression is associated with colorectal adenoma-adenocarcinoma sequence and oncogenic signalling (2015)
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