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Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: PRMT6 is a histone methyltransferase that exhibits methylation activity toward H3-R2 and H4-R3.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PRMT6 Direct Activity Assay kit is designed to measure PRMT6activity for screening and profiling applications. The key to the PRMT6Direct Activity Assay Kit is a highly specific antibody that recognizes methylated R3residue of Histone H4. With this kit, only three simple steps on a microtiter plate arerequired for methyltransferase detection. First, S-adenosylmethionine is incubated with asample containing assay buffer and methyltransferase enzyme for one hour. Next,primary antibody is added. Finally, the plate is treated with an HRP-labeled secondaryantibody followed by addition of the HRP substrate to produce chemiluminescence thatcan then be measured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with a 96-well plate precoated with histone H4 peptide substrate, the antibody against methylated arginine3 residue of Histone H4, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffe
Uniprot: Q96LA8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon S.C., et al. 2005. Genome Biology 6:227.
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