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Encompassing Amino Acids: 61-394(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-5 ng methyltransferase PRMT8) added to the wells coated with the substrate. Incubate at RT for 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally,add HRP chemiluminescent substrates and read luminesence.
Background: Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
Description: Human PRMT8 (protein arginine methyltransferase 8), GenBank Accession No. NM_019854, a.a. 61-394(end) with N-terminal His-FLAG-tag, MW=40.8 kDa, expressed in Sf9 cells using a Baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 3 mM DTT, 20% glycerol
Genbank: NM_019854
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-FLAG-tags
Uniprot: Q9NR22
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Bedford MT., et al. J Biol Chem. 2007 Dec 14;282(50):36444-53.
2. Taneda T., et al Brain Res. 2007 Jun 25;1155:1-9.
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