This ELISA incorporates some improvements over the Cat. CYGF050 and F050H kits and is designed to better detect the newer, unnatural constructs of Protein A such as the MabSelect SuReTM ligand sold by GE Healthcare. If you are using a recombinant form of Protein A with very significant structural differences from natural Protein A such as MabSelect SuReTM you should use the Catalog CYGF400 kit. Since this kit has been released, 2 newer Protein A assays, known as Mix-N-Go methods, have been developed. These new methods eliminate the sample boiling and centrifugation steps. Instead, a special sample treatment reagent is used that allows for accurate detection of Protein A in the presence of your sample antibody. The Cat. CYGF600 kit uses standards calibrated against natural and conserved recombinant forms of Protein A. The Cat. CYGF610 kit uses standards calibrated to unnatural constructs of Protein A such as GE Healthcare's MabSelect SuReTM.
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