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Encompassing Amino Acids: 1-436
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.
Background: Protein tyrosine phosphatase nonreceptor, type 1 (PTPN1) is the founding member of the protein tyrosine phosphatase (PTP) family. PTPN1 catalyze the hydrolysis of the phosphate monoesters specifically from tyrosine residues. PTPN1 is known to be involved in signaling pathways that regulate a variety of cellular processes including cell growth and oncogenic transformation. PTPN1 also has been shown to act as a negative regulator of insulin signaling. PTPN1 has also been reported to dephosphorylate epidermal growth factor receptor kinase, which implicated the role of this phosphatase in cell growth control and cell response to interferon stimulation.
Description: Protein tyrosine phosphatase PTP1B, also known as PTPN1 (GenBank accession number M31724), full length, (a.a. 1-436), with N terminal GST-tag, MW=76 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 18 mM glutathione, and 3 mM DTT.
Genbank: M31724
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P18031
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Frangioni et al., Cell, 1992, 68: 545.
2. M. Elchebly et al., Science 1999, 283: 1544.
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