Background: Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research, embryology and plant sciences, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.
Concentration: 1mg/ml
Source: Wild type recombinant GFP (from A.victoria) expressed in E.coli and purified by high resolution gel filtration.
Specificity: The purity of the recombinant GFP (wild type) protein is >98%, confirmed by SDS-polyacrylamide gel electrophoresis, specific absorbance peaks, HPLC and A397/280 ratios.
Formulation: Each vial contains 100µg 1mg/ml purified recombinant GFP (wild type) in 10 mM TRIS buffer pH 8.0, containing 0.02% sodium azide.
References: Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
UniProt: UniProt: P42212 (A.victoria)
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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