Clone: Polyclonal
Background: Desmin is a 53 kDa intermediate filament protein and exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development and muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of desmin and vimentin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
Source: K5 is a Rabbit Serum directed anti Chicken gizzard muscle desmin. Desmin was purified from a crude tissue preparation by prepaRative gel electrophoresis as described in reference 1.
Specificity: K5 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors.
Formulation: Each vial contains 250 ul Rabbit polyclonal antiSerum containing 0.09% sodium azide.
References: 1. Ramaekers FC, Puts JJ, Moesker O, Kant A, Huysmans A, Haag D, Jap PH, Herman CJ, Vooijs GP (1983). Antibodies to intermediate filament proteins in the immunohistochemical identifiCation of Human tumours: an overview. Histochem J, 15, 691-713 2. Quax W, Van den Broek L, Egberts WV, Ramaekers F, Bloemendal H (1985). Characterization of the Hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer. Cell, 43, 327-38. 3. Pieper FR, Slobbe RL, Ramaekers FC, Cuypers HT, Bloemendal H (1987). Upstream regions of the Hamster desmin and vimentin genes regulate expression during in vitro myogenesis. EMBO J, 6, 3611-8. 4. Krimpenfort PJ, Schaart G, Pieper FR, Ramaekers FC, Cuypers HT, Van den Heuvel RM, Vree Egberts WT, van Eys GJ, Berns A, Bloemendal H (1988). Tissue-specific expression of a vimentin-desmin hybrid gene in transgenic mice. EMBO J, 7, 941-7. 5. van den Heuvel RM, van Eys GJ, Ramaekers FC, Quax WJ, Vree Egberts WT, Schaart G, Cuypers HT, Bloemendal H (1988). Intermediate filament formation after transfection with modified Hamster vimentin and desmin genes. J Cell Sci, 88, 475-82. 6. Pieper FR, Schaart G, Krimpenfort PJ, Henderik JB, Moshage HJ, van de Kemp A, Ramaekers FC, Berns A, Bloemendal H (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol, 108, 1009-24. 7. Schaart G, Viebahn C, Langmann W, Ramaekers F (1989). Desmin and titin expression in early postimplantation Mouse embryos. Development, 107, 585-96. 8. Raats JM, Pieper FR, Vree Egberts WT, Verrijp KN, Ramaekers FC, Bloemendal H (1990). Assembly of amino-terminally deleted desmin in vimentin–free cells. J Cell Biol, 111, 1971-85. 9. Council, L., Hameed, O. (2009). Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma Modern Pathology 22, 639-50.
UniProt: P17661
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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