Background: Autotaxin (ATX), a tumor cell motility- stimulating protein is an ecto/exo-enzyme with significant homology to the plasma cell membrane differentiation antigen PC-1. ATX is a 99kDa core protein and may run as a 125-kDa glycoprotein when isolated from the human melanoma cell line (A2058). ATX, like PC-1 can hydrolyze the type I phosphodiesterase substrate p-nitrophenyl thymidine-5'-monophosphate. Autotaxin has a novel motility- regulating function for this class of ecto/exo-enzymes.
Positive Control: Human brain lysate (Cat. No. X1633C).
Immunogen: Synthetic peptide derived from the autotaxin protein.
Purification Method: Ammonium Sulfate Precipitation.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: [1] Murata J., Lee H.Y., Clair T., Krutzsch H.C., Arestad A.A., Sobel M.E., Liotta L.A., Stracke M.L.; cDNA cloning of the human tumor motility-stimulating protein, autotaxin, reveals a homology with phosphodiesterases.; J. Biol. Chem. 269:30479-30484(1994).
[2] Lee H.Y., Murata J., Clair T., Polymeropoulos M.H., Torres R., Manrow R.E., Liotta L.A., Stracke M.L.; Cloning, chromosomal localization, and tissue expression of autotaxin from human teratocarcinoma cells.; Biochem. Biophys. Res. Commun. 218:714-719(1996).
[3] Kawagoe H., Soma O., Goji J., Nishimura N., Narita M., Inazawa J., Nakamura H., Sano K.;
Molecular cloning and chromosomal assignment of the human brain-type phosphodiesterase I/nucleotide.; Genomics 30:380-384(1995).
[4] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
UniProt: Q13822.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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