Background: Cyclin-Dependent Kinase 2 (CDK2), also known as p33cdk2 is expressed earlier in the cell cycle than is p34cdc2. Like p34cdc2, p33cdk2 associates with cyclin A in human cells. However, kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.
Positive Control: CDK2 expressed in cells using Baculovirus as vector.
Purification Method: Ammonium Sulfate Precipitation.
Source: Rabbits were immunized with a synthetic peptide derived from the phosphorylated human cdk2 protein.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Coates, S., et al. 'A new pathway for mitogen-dependent cdk2 regulation uncovered in p27(Kip1)-deficient cells.' Curr. Biol. 1999, 9, 163-173
2. Lacy, S. & Whyte, P. 'Identification ofa p130 domain mediating interactions with cyclin A/cdk2 and cyclin E/cdk2 complexes.' Oncogene 1997, 14, 2395-2406.
UniProt: P24941.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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