Background: Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFR , which is a cell surface receptor for GDNF1,2. The cDNAs encoding GDNFR from human, rat, chicken and mouse have been cloned recently1-5. GDNFR was also termed Ret ligand 1 (RETL1) or TGF- -related neurotrophic factor receptor 1 (TrnR1) and nominated as GFR -1 recently5-7. GFR -1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFR and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.
Positive Control: Human brain tissue lysate.
Immunogen: Synthetic peptide corresponding to amino acids 369 to 382 of human GFR -1.
Purification Method: DEAE Purification.
Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative.
References: 1. Jing, S., et al. 'GDNF-induced activation of the Ret protein tyrosien kinase is mediated by GDNFR-α, a novel receptor for GDNF.' Cell, 1996, 85, 1113-1124.
2. Treanor, J.J.S., et al. 'Characterization of a multicomponent receptor for GDNF.' Nature 1996, 382, 80-83.
3. Sanicola, M., et al. 'Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins.' Proc. Natl. Acad. Sci. USA 1997, 94, 6238-6243
4. Buj-Bello, A., et al. 'Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase.' Nature 1997, 387, 721-724
5. Dey, B.K., et al. 'Cloning of a novel murine isoform of the glial cell line-derived neurotrophic factor receptor.' Neuroreport 1998, 9, 37-42
6. Baloh RH, et al. 'TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret.' Neuron 1997, 18, 793-802
7. Nomenclature Committee. Nomenclature of GPI-linked receptors for the GDNF ligand family. GFRα.. Neuron 1997, 19, 485.
UniProt: P56159.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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