Background: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN)1, play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNR , which is a cell surface receptor for NTN2,3. The cDNAs encoding NTNR from human, rat, chicken, and mouse have been cloned recently2-7. NTNR was also termed GDNFR , Ret ligand 2 (RETL2) or TGF- -related neurotrophic factor receptor 2 (TrnR2)4-7 and nominated as GFR -2 recently8. GFR -2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFR -2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function.
Positive Control: HeLa whole cell lysate.
Immunogen: Synthetic peptide corresponding to amino acids 377 to 391 of human GFR -2. This sequence is identical to those of the mouse and rat.
Purification Method: Antigen Immunoaffiinity Purification.
Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative.
References: 1. Kotzbauer, P.T., et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996, 384, 467-470
2. Klein, R.D., et al. A GPI-linked protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997, 387, 717-721
3. Buj-Bello, A., et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997, 387, 721-724
4. Baloh, R.H., et al. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997, 18, 793-802
5. Sanicola, M., et al. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc Natl Acad Sci USA 1997, 94, 6238-6243
6. Suvanto, P., et al. Cloning, mRNA distribution and chromosomal localisation of the gene for glial cell line-derived neurotrophic factor receptor beta, a homologue to GDNFR-alpha. Hum Mol Genet 1997, 6, 1267-1273
7. Wang, C.Y., et al. Cloning and characterization of glial cell line-derived neurotrophic factor receptor-B: a novel receptor for members of glial cell line-derived neurotrophic factor family of neurotrophic factors. Neuroscience 1998 Mar, 83(1), 7-14
8. Nomenclature Committee. Nomenclature of GPI-linked receptors for the GDNF ligand family. GFRα. Neuron 1997, 19, 485.
UniProt: O00451.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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