Background: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF- -induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
Positive Control: SW1353 whole cell lysate.
Immunogen: Synthetic peptide corresponding to amino acids 1356 to 1375 of human ASK1 protein. Immunogen sequence differs in mouse by last two amino acids.
Purification Method: Antigen Immunoaffiinity Purification.
Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative.
References: 1. Ichijo, H., et al., 'Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways.' Science 1997, 275, 90-94
2. Wang, X.S., et al., 'Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase.' J. Biol. Chem. 1996, 271, 31607-31611
3. Tobiume, K., et al., 'Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1.' Biochem. Biophys. Res. Commun. 1997, 239, 905-910.
UniProt: Q99683.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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