Background: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis.Netrin 1Õs main receptor is DCC (deleted in colorectal cancer- a putative tumor suppressor gene whose expression is lost in numerous cancers). DCC belongs to the so-called family of dependence receptors. These receptors induce apoptosis when their ligand is absent, thus conferring a state of cellular dependence on Netrin 1 availability. The DCC/netrin-1 duo regulates neuron survival during nervous system development. The mechanism(s) by which Netrin 1/ DCC triggers cell death are still unknown. The localization of DCC to lipid rafts appears to be a prerequisite for its proapoptotic activity.
Positive Control: Antibody tested on MDCKII cell lysates.
Immunogen: Synthetic peptide derived from the human Netrin 1 protein
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
References: 1. Nguyen, A., et al. 'Netrin-1 induces angiogenesis via a DCC-dependent ERK 1/2-eNOS feed-forward mechanism.' Proc. Natl. Acad. Sci. USA, 2006, 103, 6530-65352. Shekarabi, M., et al. 'Deleted in colorectal cancer binding netrin-1 mediates cell substrate adhesion and recruits Cdc42, Rac1, Pak1, and N-WASP into an intracellular signaling comlex that promotes growth cone expansion.' J. Neurosci. 2005, 25, 3132-31413. Jarjour, A.A., et al. 'Netrin-1 is a chemorepellent for oligodendrocyte precursor cells in the embryonic spinal cord.' J. Neurosci. 2003, 23, 3735-37444. Furne C, et al. 'The dependence receptor DCC requires lipid raft localization for cell death signaling.' Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4128-33. Epub 2006 Mar 6. 5. Furne C, Mehlen P. 'The deadly rafts: when lipid rafts regulate dependence receptors.' Med Sci (Paris). 2006 Nov;22(11):909-10.6. Nakamura F, Takei K, Goshima Y. 'Signaling mechanism connecting axon guidance molecules and growth cone cytoskeleton.' Tanpakushitsu Kakusan Koso. 2006 May;51(6 Suppl):733-41. 7. Chang C, et al. 'MIG-10/lamellipodin and AGE-1/PI3K promote axon guidance and outgrowth in response to slit and netrin.' Curr Biol. 2006 May 9;16(9):854-62. Epub 2006 Apr 20. PMID: 166185418. Xie Y, et al. 'DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation.' J Biol Chem. 2006 Feb 3;281(5):2605-11. Epub 2005 Nov 30.
UniProt: NP_004813
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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