Background: Genetic studies in Drosophila have revealed a novel secreted antagonist for Wingless (Wg) signaling, referred to as Notum/Wingful (Wf). Wf/Notum encodes a protein of 430 amino acid residues, which is related to plant pectin acetylesterases of the / -hydrolase superfamily. The main target of Notum action appears to be cell surface proteoglycans Dally and/or Dally-like (Dlp). Notum governs the extracellular Wg morphogen distribution via Dally and Dlp. Also, like some vertebrate Wnt antagonists, notum expression is activated by Wg signaling, revealing the existence of a feedback control mechanism in which Wg modifies its own gradient distribution. In vertebrate, proteoglycans related to Dally and Dlp likely play important roles in Wnt signaling which encodes a glypican that is required for Wnt-11 signaling. A single ortholog of Notum exists in the mouse and the human genome, implying that a similar regulatory mechanism may exist in vertebrates.
Positive Control: HepG2 cell lysate.
Purification Method: Ammonium Sulfate Precipitation.
Source: Rabbits were immunized with recombinant full-length notum protein from mouse.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Kozmik, Z., et al. 'Characterization of mammalian orthologues of the Drosophila osa gene: cDNA cloning, expression, chromosomal localization, and direct physical interaction with Brahma chromatin-remodeling complex.' Genomics, 2001, 73, 140-148
2. Riese, J., et al. 'LEF-1, a nuclear factor coordinating signaling inputs from wingless and decapentaplegic.' Cell, 1997, 88, 777-787
3. Giraldez, A.J., et al. 'HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient.' Dev. Cell, 2002, 2, 667-676.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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