Background: The p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains. However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions. p73 is expressed in either a full-length form ( ) (MW ~80kDa) or a shorter ( ) (MW ~70kDa) mRNA variant, with exon 13 spliced out.
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Positive Control: COS monkey kidney cells and IM9 human myeloma cells.
Immunogen: GST-fused recombinant protein derived from the C terminal of the p73 protein.
Purification Method: Ammonium Sulfate Precipitation.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Zeng, X., et al. 'p300 does not require its acetylase activity to stimulate p73 function.' J. Biol. Chem. 2001: 276, 48-52.
2. Dobbelstein M, Roth J: The large T antigen of simian virus 40 binds and inactivates p53 but not p73. J Gen Virol 1998;79:3079-3083.
3. Higashino F, et al: Adenovirus e4orf6 oncoprotein modulates the function of the p53-related protein, p73. Proc Natl Acad Sci U S A 1998 Dec 22;95(26):15683-15687.
4. Lo Cunsolo C, et al: Refined chromosomal localization of the putative tumor suppressor gene TP73. Cytogenet Cell Genet 1998;82(3-4):199-201.
5. Kovalev S, et al: Expression level, allelic origin, and mutation analysis of the p73 gene in neuroblastoma tumors and cell lines. Cell Growth Differ 1998 Nov;9(11):897-903.
6. Zhu J, et al: The potential tumor suppressor p73 differentially regulates cellular p53 target genes. Cancer Res 1998 Nov 15;58(22):5061-5065.
7. De Laurenzi V, et al: Two new p73 splice variants, gamma and delta, with different transcriptional activity. J Exp Med 1998 Nov 2;188(9):1763-1768.
8. Nimura Y, et al: p73, a gene related to p53, is not mutated in esophageal carcinomas. Int J Cancer 1998 Nov 9;78(4):437-440.
9. Marin MC et. al. Viral oncoproteins discriminate between p53 and the p53 homolog p73. Molecular and Cellular Biology, 1998;18(11):6316-6324.
UniProt: O15350.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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