Background: Vascular endothelial growth factor and type I collagen inducible protein (VCIP), also known as phosphatidic acid phosphatase 2b (PAP2b), was originally identified in a functional assay of angiogenesis. VCIP/PAP2b exhibits an ArgÐGlyÐAsp (RGD) cell adhesion sequence. Immunoprecipitation and fluorescence-activated cell sorting analyses demonstrated that VCIP-RGD is exposed to the outside of the cell surface. Retroviral transduction of VCIP can induce cell aggregation and cellÐ cell interactions, modestly increase p120 catenin expression and promote activation of the Fak, Akt and GSK3§ protein kinases. Expression of VCIP promoted adhesion, spreading and tyrosine phosphorylation of Fak, Shc, Cas and paxillin in endothelial cells. PAP2b/VCIP is expressed in close proximity to vascular endothelial growth factor, von Willebrand factor and {alpha}v§3 integrin in tumor vasculatures.
Positive Control: Human brain lysate (Cat. No. X1633C).
Immunogen: Synthetic peptide derived from the phosphatidic acid phosphatase 2B protein.
Purification Method: Antigen Immunoaffiinity Purification.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: [1] Kai M., Wada I., Imai S.-I., Sakane F., Kanoh H.; Cloning and characterization of two human isozymes of Mg2+-independent phosphatidic acid phosphatase.; J. Biol. Chem. 272:24572-24578(1997).
[2] Roberts R., Sciorra V.A., Morris A.J.; Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.; J. Biol. Chem. 273:22059-22067(1998).
[3] Humtsoe J.O., Feng S., Thakker G.D., Yang J., Hong J., Wary K.K.; Regulation of cell-cell interactions by phosphatidic acid phosphatase 2b/VCIP.; EMBO J. 22:1539-1554(2003).
[4] Leung D.W., Tompkins C.K.; Molecular cloning of and expression of an isoform of human phosphatidic acid phosphatase cDNA.; Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
[5] Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A.; Large-scale concatenation cDNA sequencing.;
Genome Res. 7:353-358(1997).
[6] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
[7] Burnett C., Makridou P., Hewlett L., Howard K.; Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity.; BMC Biochem. 5:2-2(2004).
UniProt: O14495.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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