Background: Endothelial Cell Differentiation Gene-8 (EDG-8) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-8 is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG-8 is expressed in oligodendrocytes and fibrous astrocytes in the rat brain.
Positive Control: RH7777 cells transfected with full length EDG8 protein.
Purification Method: Ammonium Sulfate Precipitation.
Source: Rabbits were immunized with a unique synthetic peptide derived from the C-terminus of the human EDG-8 protein.
References: 1. Im, D.S., et al. 'Characterization of a novel sphingosine-1-phosphate receptor, Edg-8.' J. Biol. Chem. 2000, 275, 14281-14286
2. Malek, R.L., et al. Nrg-1 belongs to the EDG family of G-protein coupled sphingosine-1-phosphate receptors. J. Biol. Chem. 2000, epub ahead of print.
3. Hla, T., et al. Sphingosine-1-phosphate signaling via the EDG-1 family of G-protein coupled receptors. Ann. N.Y. Acad. Sci. 2000, 905, 1624
4. Pyne, S. & Pyne, N.J. Sphingosine-1-phosphate signalling in mammalian cells. Biochem. J. 2000, 349, 385-402.
UniProt: Q9H228.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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