Background: Human Ssu72 is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/termination machinery. The human Ssu72 is located on Chromosome 1p36 and seems to represent a single gene. It was identified as a pRb binding factor and was also shown to interact with TFIIB and yeast PTA1. Rabbit polyclonal antibodies were raised against SSU72 fusion protein. The ABs recognize a single band of ~30 Kda on immunoblots of various human cell lines but do not work well on mouse tissues.
Positive Control: MCF-7 and Cos-7 cells.
Immunogen: Full length recombinant human Ssu72 protein.
Purification Method: Ammonium Sulfate Precipitation.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. St-Pierre, B., et al. 'Conserved and specific functions of mammalian ssu72' Nucleic Acids Res. 2005, 33, 464-477
2. Krishnamurthy, S., et al. 'Ssu72 is an RNA polymerase II CTD phosphatase' Mol. Cell 2004, 14, 387-394
3. Steinmetz, EJ., Brow, DA. 'Ssu72 protein mediates both poly(A)-coupled and poly(A)-independent termination of RNA polymerase II transcription' Mol Cell Biol 2003, 23, 6339-6349.
UniProt: Q15526.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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