Background: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (4,5). TLR7, like TLRs 3, 8, and 9, is localized in intracellular acidic compartments such as the phagolysosome (6) and will recognize some single-stranded RNA viruses such as vesicular stomatitis virus (VSV) and influenza virus (7). Activation of TLR7 by VSV results in stimulation of the immune response including IFNA secretion, suggesting the importance of TLR7 in virus recognition.
Positive Control: Positive Control-Daudi Cell Lysate
Located in the membrane, TLR7 is detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
Immunogen: Rabbit polyclonal TLR7 antibody was raised against a peptide corresponding to 14 amino acids near the middle of mouse TLR7.
Purification Method: Antigen Immunoaffiinity Purification.
Formulation: Provided as solution in phosphate buffered saline with 0.02% sodium azide.
References: 1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
2) Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
3.) Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
4) O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
5) McGettrick AF and O’Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol Imm. 2004; 41:577-82.
6) Nishiya T and DeFranco AL. Ligand-regulated chimeric receptor approach reveals distinctive subcellular localization and signaling properties of the Toll-like receptors. J. Biol. Chem. 2004; 279:19008-17.
7) Lund JM, Alexopoulou L, Sato A, et al. Recognition of single-stranded RNA viruses by Toll-like receptor 7. Nature 2002; 420:329-333.
8) UniProtKB/Swiss-Prot entry Q9NYK1, http://www.expasy.org/uniprot/Q9NYK1, Accesseer February 9, 2007.
UniProt: Q9NYK1.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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