Clone: Polyclonal
Background: The reactivity of the antiserum is directed to all known isotypes of the rat immunoglobulin system (classes, subclasses and light chain types) including antibodies to common determinants, to class and subclass specific determinants, and to the surface determinants of the common Fab portion as tested by immunoelectrophoresis and double radial immunodiffusion. It does not react with any non-Ig protein in rat serum. To identify rat immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Source: Purified polyclonal rat IgG, including all subclasses, and pools of homogenous immunoglobulins. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Rat immunoglobulins, heavy and light chains.
Formulation: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
English
Deutsch
