Background: The Kv1.5 potassium channel is a voltage-gated channel protein which belongs to the delayed rectifier class and to the Shaker potassium channel subfamily which includes Kv1.1, Kv1.2, Kv1.3 and Kv1.4. Potassium channels are mainly found in plasma membranes but are not generally distributed over the cell surface. Potassium channels catalyze the rapid permeation of potassium ions while rejecting biologically abundant potential competitors such as sodium, calcium and magnesium. Ion selectivity and high through put rate of potassium channels is accomplished by precise co-ordination of dehydrated potassium by the protein and multiple ion occupancy within the permeation pathway. All potassium channels carry out the formation of a transmembrane leak specific for potassium ions. Since cells almost universally maintain cytoplasmic potassium concentrations higher than those extracellularly, the opening of a potassium channel implies a negative ongoing change in electrical voltage across the cell membrane. This may result in termination of the action potential of electrically excitable cells including nerve, muscle and pancreatic beta cells. In nonÐexcitable cells, potassium channels play important roles in the cellular potassium recycling required for electrolyte balance effected by the renal epithelium.
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Positive Control: Rat brain lysate.
Purification Method: Ammonium Sulfate Precipitation.
Source: Rabbits were immunized with a synthetic peptide derived from the rat Kv1.5 potassium channel conjugated to KLH.
References: 1. Swanson, R et.al 'Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain' Neuron 4 (6), 929-939 (1990)
2. Roberds,S.L. et.al 'Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart' Proc. Natl. Acad. Sci. U.S.A. 88 (5), 1798-1802 (1991)
3. Mori, Yet.al 'The transcription of a mammalian voltage-gated potassium channel is regulated by cAMP in a cell-specific manner' J. Biol. Chem. 268 (35), 26482-26493 (1993)
4. Li, H. et.al., 'Functional expression of a GFP-tagged Kv1.5 alpha-subunit in mouse ventricle.' Am J Physiol Heart Circ Physiol. 281(5):H1955-67 ( 2001).
UniProt: P22460.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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