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Applications: The RARα Reporter (Luc)-HEK293 Cell Line is designed for monitoring the activity of retinoic acid receptor alpha (RARα). Monitor RARα-regulated pathway activity Screen agonists or antagonists of RARα.
Background: RAR belongs to a family of nuclear receptors and has three subtypes, RARα, RARβ, and RARγ. RAR heterodimerizes with RXR (retinoid X receptor) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes.
Biological Activity: This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid. The expression of RARα is confirmed by Western blotting.
Description: The RARα Reporter (Luc)-HEK293 Cell Line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (NM_000964).
Format: aqueous solution containing DMSO
Genbank: NM_000964
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.
Uniprot: P10276
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Petkovich, M, et al. Nature (1987) 330(6147): 444-450. 2. Allenby, G, et al. Proc. Natl. Acad. Sci. USA (1993) 90(1): 30-34.
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