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Applications: The RARβ Reporter (Luc)-HEK293 cell line is designed for monitoring the activity of RARβ.
Monitor RARβ-regulated pathway activity
Screen agonists or antagonists of RARβ.
Biological Activity: This cell line is functionally validated for the response to the stimulation of all-trans retinoic acid. The expression of RARβ is confirmed by Western blotting.
Description: The RARβ Reporter (Luc)-HEK293 cell line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARβ (accession # P10826-2).
Format: aqueous solution containing DMSO
Genbank: P10826-2
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~2 X 10^6 cells in 1 ml of 10% DMSO.
Uniprot: P10826-2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Petkovich, M, et al. Nature (1987) 330(6147): 444-450.
2. Allenby, G, et al. Proc. Natl. Acad. Sci. USA (1993) 90(1): 30-34.
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