Clone: 33B12
Background: The Forssman glycolipid is a glycosylceramide possessing a neutral pentasaccharide head group, referred to as the Forssman antigen (Fa) and is a member of the globoseries glycolipid family. Fa has been identified in a number of mammals and exhibits heterogeneity with respect to developmental and cell-type expression among species. Some studies have reported the presence of the Fa in certain Human embryonic and tumor cells.The majority of Human individuals have undetectable levels of Fa. Gastric and colonic tumors from Fa-negative patients, however, contained relatively high levels of Fa. Moreover, compared with normal lung tissue, many lung cancers contained elevated levels of Fa. These observations arouse interest in Fa for a possible role as a Human tumor antigen.
Source: The antibody 33B12 is a Rat anti-Forrsman glycosphingolipid (FGL) antibody derived by fusing Mouse myeloma cells with spleen cells from a Rat immunized with Mouse mammary tumors.
Specificity: Clone 33B12 and 117C9 detect overlapping epitopes on the Forssman glycolipid hapten (GalNA1- c3aG alNAcD1 -3GalaI-4GalB1-4GlcBl-lCer). Clone 33B12 reacts with the terminal sugar sequence GalNAcd-3GalNAc and is specific for Forssman antigen.
Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
References: 1. Sonnenberg A, van Balen P, Hengeveld T, Kolvenbag GJ, Van Hoeven RP, Hilgers J., Monoclonal antibodies detecting different epitopes on the Forssman glycolipid hapten. J Immunol. 1986 Aug 15;137(4):1264-9
UniProt: Q8VI38
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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