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Encompassing Amino Acids: 352-620(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Z-Lyte® (Invitrogen) screening protocol and condition were used. Enzyme reaction was conducted at 30°C for 1 hours with 2 µM Tyr 01 Peptide in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour incubation, diluted Development Reagent B was added.
Description: Rat recombinant IL2-inducible T-cell kinase (ITK), also known as Lyk and TSK (GenBank Accession #NM_001108825), a.a. 352-620(end) with N-terminal GST-tag, MW= 57 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
Genbank: NM_001108825
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: D4A7W7
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hawkins, J. and Marcy, A. (2001). Protein Expr. Purif. 22 (2): 211-9.
2. Kosaka,Y., et al. (2006). Trends Immunol. 27 (10): 453-60.
3. Tanaka, N., et al. (1993). FEBS Lett. 324 (1): 1-5.
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