Usage: This ELISA kit applies to the in vitro quantitative determination of Rat LF/LTF concentrations in Serum, plasma and other biological fluids.
Tested Sample Types: Serum, plasma and other biological fluids
Assay Time: 3.5h
Sensitivity: 7.5 pg/mL
Detection Range: 12.5-800 pg/mL
UNIProt ID: D3ZAB1
Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LF/LTF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LF/LTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LF/LTF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat LF/LTF. You can calculate the concentration of Rat LF/LTF in the samples by comparing the RLU value of the samples to the standard curve.
Cross reactivity: This kit recognizes Rat LF/LTF in samples.No significant cross-reactivity or interference between Rat LF/LTF and analogues was observed