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Applications: Study enzyme kinetics and use in high throughput screening (HTS) applications.
Description: The RdRp (SARS-CoV-2) TR-FRET Assay Kit is designed to measure the activity of the SARS-CoV-2 RNA-dependent RNA Polymerase (RdRp) for screening and profiling applications. RdRp operates as a complex of NSP12, NSP7, and NSP8 proteins. As a crucial enzyme in the life cycle of coronaviruses, RdRp represents one of the most promising druggable targets for SARS-CoV-2. The assay measures the direct incorporation of biotinylated ATP into the double-stranded RNA substrate. The increase in TR-FRET signal is proportional to the amount of ATP incorporated in the RNA. With this homogeneous kit, only two steps are required for RdRp activity detection. First, a test compound is incubated with the enzyme in the reaction mixture. Next, Dye-labeled Acceptor and Eu-labeled Antibody Donor are added, followed by reading of the TR-FRET signal.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The RdRp (SARS-CoV-2) TR-FRET Assay Kit comes in a convenient 384-well format, with Digoxigenin-labeled RNA duplex, biotinylated ATP, RdRp assay buffer (2 components plus DTT), and purified RdRp complex.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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