Abbreviation: LacI
Target Synonym: Lactose operon repressor;LacI
Target Species: E.coli
Expression Host: E.coli
Fusion Tag: C-His
UNIProt ID: P03023
Background: Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
Sequence: Met1-Gln360
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Supplied as a 0.2 um filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
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