Abbreviation: Caspase-8;CASP8
Target Synonym: CASP8_MOUSE;Caspase-8;Casp8;EC:3.4.22.61
Target Species: Mouse
Expression Host: E.coli
Fusion Tag: N-His
UNIProt ID: O89110
Background: Caspase-8 (Cysteine-aspartic acid protease 8/Casp8a; also named MCH5, FLICA and MACH alpha 1) is a 28 kDa member of the peptidase C14A family of enzymes. It is widely expressed and is considered an initiating caspase for the apoptotic cascade. Caspase-8 acts on a wide variety of substrates, including procaspases?3, 4, 6, 7, 9 and 10, c?FLIPL and procaspase-8 itself. Human procaspase?8a is a 54?56 kDa, 479 amino acid (aa) protein. It contains two N?terminal death domains (aa 1?177), followed by a catalytic site that utilizes His317Gly318 plus Cys360. Normally, it is an inactive, cytosolic monomer. But following death?domain (DD) containing receptor oligomerization, Caspase?8 is recruited to the death-inducing signaling complex (DISC) that forms around the death domains of the oligomerized receptor.
Sequence: Ser 219-Gly 376
Purity: > 95 % as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Endotoxin: Please contact us for more information.
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