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Applications: Substrate for histone methyltransferase or acetyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases and acetyltransferases for drug discovery and HTS applications. Since these nucleosomes were prepared in E
Background: Chromatin, the material into which genomic DNA is packaged in eukaryotes, is a very dynamic structure. The smallest subunit of chromatin is the nucleosome, consisting of ~147 base pairs of DNA wrapped around an octamer of core histone proteins. The histone octamer is composed of a central heterotetramer of histones H3 and H4, flanked by two heterodimers of histones H2A and H2B. Each nucleosome is separated by 10 to 60 bp of linker DNA. The resulting nucleosomal array constitutes a chromatin fiber of about 10 nm in diameter. This arrangement is folded into more condensed fibers (about 30 nm) that are stabilized by binding of a linker histone (Histone H1) to each nucleosome core. Such 30 nm fibers are then condensed in vivo to form thicker interphase fibers or the most highly compacted metaphase chromosome structures
Description: Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a. 2-130(end)), H2B (a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Each H2B has a C-terminal Cys added as a biotinylation site. Total histone MW (before biotinylation) =113.8 kDa.
Format: Aqueous buffer solution
Formulation: 20 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, and 20% glycerol.
Genbank: NM_033445, NM_003528, NM_003532, NM_003549
Host Cell Line: E. coli
Storage Stability: Stable at -80°C for at least 6 months from date of receipt, when stored as directed
Tags: N-terminal His-tag, Biotin
Uniprot: Q7L7L0, Q16778, P68431, P62805
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20,5(1):11.
2. Huang, H., et al., Biosystems 2012 Aug,109(2):214-9.
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