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Applications: Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.
Description: Recombinant human nucleosome consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A, encompassing amino acids 2-130(end), H2B encompassing amino acids 2-126(end), H3 encompassing amino acids 2-137(end), and H4 encompassing amino acids 2-103(end). Each histone construct contains an N-terminal His-tag (6xHis). The recombinant complex was affinity purified.
Format: Aqueous buffer solution
Formulation: 20 mM Tris, pH 7.5, 1 mM EDTA, 1 mM DTT, and 20% glycerol
Genbank: Histone H2A: NM_033445; Histone H2B: NM_003528; Histone H3: NM_003532; and Histone 4: NM_003548
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Histone H2A: Q7L7L0; Histone H2B: Q16778; Histone H3: P68431; Histone H4: P62805
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11.
2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.
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