Application Note: RFP Control is suitable as a control for polyclonal or monoclonal Anti-RFP in immunological assays. The molecular weight of RFP (calculated) is 27 kDa, but in a gel it is expected to run at approximately 28-30 kDa. Recombinant RFP has been tested in SDS-page, dot blot, and western blot.
Concentration Value: 1.0 mg/mL
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Physical State: Liquid (sterile filtered)
Purity and Specificity: Recombinant RFP was synthesized with an amino terminal 6XHIS tag and was expressed in E. coli. A 3 step process was used to purify the protein: a partitioning separation using t-butanol, followed by hydrophobic interaction chromatography (HIC), and lastly IMAC nickel chromatography. Analysis by SDS-PAGE resulted in a pattern consistent with purified RFP and was estimated to be greater than 90% pure.
Background: Recombinant RFP (wild type, sequence corresponding to RFP, Discosoma spp.) is intended for use as a control when using polyclonal or monoclonal Anti-RFP in immunological assays, in fluorometry, and in fluorescence activated cell sorting (FACS). Rockland offers multiple purified hosts and conjugates of Anti-RFP that can detect RFP by ELISA and western blot. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA is well suited to titrate RFP in solution when used in combination with unconjugated Anti-RFP antibody. This product can be used as a control in western blots.
Low Endotoxin: No
Other: User Optimized
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