CloneID: F9
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was selected from a synthetic human scFv library encoding HA-tagged scFv clones through five rounds of biopanning against synthetic and recombinant Pep27.
Origin Pub PMID: 29753735
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human scFv antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: B2IMR9
Specificity Statement: This antibody is specific for the Streptococcus pneumoniae Pep27. The epitope recognized by this antibody is located within the residues 18-27, with key residues crucial for the binding being Y24, R26, and K27, as identified through alanine scanning and further supported by molecular docking analyses. Streptococcus pneumoniae Pep27 is a small, secreted peptide consisting of 27 amino acids initially described as a "death signal peptide".
Application Notes (Clone): The dissociation constant (Kd) of the original human scFv version of this antibody was 0.5 µM as measured in an ELISA. This antibody specifically detected Pep27 in an immunoblot and in an environment mimicking in vivo conditions, demonstrated in human serum (Kim et al. 2018; PMID: 29753735). This antibody was successfully converted and expressed as a Fab, scFv-Fc, scFv-CH, and IgG using an antibody reformatting vector system (AbVec) (Kim, Park, & Lee, 2019).
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