CloneID: D3H5
Antigen Long Description: This antibody was raised by immunizing mice with a recombinant apo form of G93A human SOD1
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody was specific for misfolded SOD1 and does not bind to WT SOD1.
Application Notes (Clone): The original antibody was used for an ELISA on SOD1 WT and misfolded SOD1. It only showed binding to the misfolded SOD1 protein. This antibody was used for a western blot on SOD1 WT and misfolded SOD1. This antibody was expressed in the spinal cords of mice with a SOD1 mutation simulating ALS. It showed that it delayed disease onset and extended lifespans (Patel et al., 2014; PMID:24394188). This antibody was used for immunohistochemistry on spinal cords from transgenic mice over-expressing the G93A-SOD1 mutation (Gros-Louis et al., 2010; PMID:20345765), and on SOD1G93A rat spinal cords (Pickles et al., 2016; PMID:27121871). The antibody was used for immunoprecipitation on spinal cords from transgenic mice over-expressing the G93A-SOD1 mutation. This antibody was used in vivo on G93A-SO D1 mice. It delayed the mortality on these mice (Gros-Louis et al., 2010; PMID:20345765).
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