CloneID: T3
Antigen Long Description: The original antibody was generated by immunizing mice a THC-BSA conjugate.
Origin Pub PMID: 20630472
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for Δ9-THC and its metabolites, including 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (11-nor-Δ9-THC—COOH) and 11‑hydroxy-Δ9-tetrahydrocannabinol (11-OH-Δ9-THC).
Application Notes (Clone): The binding affinity and specificity of the original version of this antibody (mouse Fab) were determined in a competitive ELISA. Structural analysis of this antibody — free and in complex with THC — was determined through crystallization. This antibody was also used to develop a sensitive, one-step, homogeneous FRET immunoassay for detecting THC and its metabolites in saliva samples (Niemi et al., 2010; PMID: 20630472) (US8518653B2). This antibody was successfully transiently expressed in N. benthamiana by using a geminiviral expression system. The plant-produced version of this antibody retained the binding activity to Δ9-THC and its metabolites and provided high sensitivity in detecting Δ9-THC in marijuana, inflorescence, and leaf extracts but not in stem, root, or non-cannabis controls (Boonyayothin et al., 2022; PMID: 35686006).
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