Presentation: Lyophilized from sterile filtered 10mM Sodium Phosphate, pH 7.5
Description: Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8a and CD8ß, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8a chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8a. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naïve CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-ß1. CHO cell-derived Recombinant Human sCD8a is a monomeric glycoprotein of 161 amino acid residues, which corresponds to the extracellular domain of CD8a. CHO cell-derived Recombinant Human sCD8a has a calculated molecular weight of 17.6 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of approximately 27-29 kDa by SDS-PAGE analysis, under reducing conditions.
Synonyms: CD8A, CD8a molecule, CD8a antigen, OKT8 T-cell antigen, Leu2 T-lymphocyte antigen, T8 T-cell antigen, T cell co-receptor, p32, CD8, Leu2, T-cell antigen Leu2
Recommended Storage: Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 1 week at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Protein Type: Recombinant
Reconstitution Instructions: Centrifuge vial prior to opening. Do not vortex. Reconstitute with H2O to 0.1-1.0 mg/ml.
Purification: Greater than 95% by SDS-PAGE gel and HPLC analyses.
Predicted Molecular Weight: 17.6 kDa
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