Formulation: 200 µg of peptide in 200 µl TBS, pH 7.4, containing 0.1% BSA and 0.02% sodium azide
Shelf life (days): 1095
Notes: To be used in conjunction with Cayman’s MC4R polyclonal antibody (Catalog No. 10006355) to block protein-antibody complex formation during immunochemical analysis of melanocortin-4 · Melanocortins are known to exert a broad array of physiological actions including 'melanogenesis, steroidogenesis, sexual function and inflammation, as well as appetite regulation and energy homeostasis'.{12351} These actions are mediated (differentially or concurrently) in part by a family of G-protein coupled receptors, the melanocortin receptors 1-5 (MC1R-MC5R). Melanocortin-4 receptor (MC4R) was first cloned in 1993 with transcripts expressed primarily in the brain.{13229} Genetic studies of mice and humans have established a critical role of MC4R in appetite regulation.{3777} Heterozygous mutations in MC4R account for 1-6% of severe cases of human obesity. Thus, MC4R has been a prime target for therapeutic intervention in obesity.{10617} All known mammalian MC4Rs are 332 amino acids in length with an estimated molecular weight of 37 kDa. The protein is heavily glycosylated and appears at multiple positions on SDS-PAGE.{13230} Cayman’s MC4R polyclonal antibody mainly detects an unglycosylated protein at 37 kDa and a glycosylated protein at 55 kDa.
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