Formulation: 200 µg of peptide lyophilized from from 200 µl water
Shelf life (days): 1095
Notes: To be used in conjunction with Cayman’s NAPE-PLD (Internal) Polyclonal Antibody (Item No. 10305) to block protein-antibody complex formation during analysis of NAPE-PLD. N-Acylethanolamines (NAEs) are involved in diverse biological processes such as inflammatory regulation, apoptosis, and tissue degeneration.{12164} In animals, NAEs are mainly biosynthesized via a membrane phospholipid-dependent pathway, which is the enzymatic hydrolysis of N-acyl-phosphatidylethanolamine (NAPE). The enzyme catalyzing this reaction is a phospholipase D (PLD) subtype selective for NAPE named N-acylphosphatidylethanolamine-hydrolysing PLD (NAPE-PLD). It has been cloned from mouse, rat, and human and is 393-396 amino acids in length, with an estimated molecular weight of 46 kDa. Both NAPE-PLD mRNA and protein activity have been detected in a wide range of tissues with the highest levels in brain, kidney, and testis.{11963} In rat, NAPE-PLD activity in the brain is low in neonates and is 15-fold higher in adults, whereas the activity remains constant in the heart during development.{13170}
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