Application Note: WB: 0.25-0.5 μg/mL. ICC/IF: 5 μg/mL. IHC-P: 2-5 μg/mL. FACS: 1-3 μg/1x10⁶ cells. ELISA: 0.1-0.5 μg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 76
Form: Liquid
Buffer (with preservative): 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, no preservatives.
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Background: Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]
Uniprot ID: Q13976
Antigen Species: Human
Immunogen: E.coli-derived human cGKI/PRKG1 recombinant protein (Position: S2-Q44).
Purification: Purified by antigen-affinity chromatography
Conjugation: Unconjugated
Full Name: protein kinase cGMP-dependent 1
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