Cytochrome C (Mitochondrial Marker)(rCYCS/1010), 0.2mg/mL

Cytochrome C (Mitochondrial Marker)(rCYCS/1010), 0.2mg/mL
SKU
BTMBNUB3641-100
Packaging Unit
100 uL
Manufacturer
Biotium

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Description: Recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i. e. cytochrome in the cytosol without heme attached) and holocytochrome (i. e cytochrome in the mitochondria with heme attached). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Conjugate: Purified, with BSA

Concentration: 0.2 mg/mL

Storage buffer: PBS, 0.05% BSA, 0.05% azide

Product Origin: Animal

Clone: rCYCS/1010

Entrez Gene ID: 54205

Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Verified AB Applications: Flow (intracellular) (verified)/IHC (FFPE) (verified)/WB (verified)

Z-Antibody Applications: Flow, intracellular (verified)/IHC, FFPE (verified)/WB (verified)
More Information
SKU BTMBNUB3641-100
Manufacturer Biotium
Manufacturer SKU BNUB3641-100
Green Labware No
Package Unit 100 uL
Quantity Unit STK
Reactivity Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Various species, Frog (Xenopus Laevis), Horse (Equine), Fruit Fly (Drosophila Melanogaster)
Clonality Recombinant
Application Western Blotting, Flow Cytometry, Immunohistochemistry
Isotype IgG1 kappa
Host Mouse
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